Journal: bioRxiv

Article Title: Simple rules govern the diversity of bacterial nicotianamine-like metallophores

doi: 10.1101/641969

Figure Lengend Snippet: Activity of CntL and CntM from Paenibacillus mucilaginosus. (A) TLC separation of reaction products incubating carboxyl-[ 14 C]-labeled with purified enzyme (PmCntL and PmCntM) and various substrates (L-His (L) or D-His (D), pyruvate (Pyr) or α-ketoglutarate (αKG), NADH or NADPH). (B) Activity profile of PmCntM using variable concentrations of pyruvate (black circle) and α-ketoglutarate (white circle) with fixed concentrations of others substrates: 0.2 mM of NADPH and 0.2 mM of xNA. The data points are means of three replicates with standard deviations. The fits are made using the Michaelis-Menten model. (C) Biosynthetic pathway for the assembly of bacillopaline from D-His, SAM and α-ketoglutarate.

Article Snippet: Paenibacillus mucilaginosus , sub sp. 1480D (from Collection DSMZ) was grown by inoculating stock bacteria into 20 mL sterile medium (TSB 1/10) at 30°C for 48h.

Techniques: Activity Assay, Labeling, Purification

Journal: Microorganisms

Article Title: The Form of N Supply Determines Plant Growth Promotion by P-Solubilizing Microorganisms in Maize

doi: 10.3390/microorganisms7020038

Figure Lengend Snippet: Shoot biomass ( A ), shoot P content ( B ), root dry matter ( C ), and total root length ( D ) of maize (cv Colisee) grown on a calcareous Loess subsoil (pH 7.6)—sand mixture (80/20% w / w ), supplied with and without (Zero) P fertilization in the form of Rock-P (RP) or soluble Ca(H 2 PO 4 ) 2 (+P) and calcium nitrate fertilization. RP variants inoculated with Pseudomonas sp. DSMZ 13134 Proradix (Pro); SP11, Vitalin SP11 (SP11); Penicillium sp. PK 112 (BFOC); and Paenibacillus mucilaginosus (Paeni). Means of four replicates. One-way ANOVA, Tukey test. Different letters indicate significant differences ( p < 0.05).

Article Snippet: Plants were inoculated with different PSMs of fungal and bacterial origin, comprising three single-strain inoculants ( Pseudomonas sp. DSMZ 13134 Proradix ® (Pro), Paenibacillus mucilaginosus (Paeni), Penicillium sp. PK 112, Biological Fertilizer OD (BFOD) and one consortium product, Vitalin SP11, with a combination of Bacillus subtilis , Pseudomonas sp., Streptomyces spp., humic acids, and extract of the seaweed, Ascophyllum nodosum .

Techniques:

Journal: Microorganisms

Article Title: The Form of N Supply Determines Plant Growth Promotion by P-Solubilizing Microorganisms in Maize

doi: 10.3390/microorganisms7020038

Figure Lengend Snippet: Shoot biomass ( A ) and total root length ( B ) of maize (cv Colisee) grown on a clay-loam, organic farming soil (pH 7.0), supplied with and without (No P) P fertilization in form of Rock-P or soluble triple superphosphate (TSP). N supply in the form of Ca-nitrate (NO 3 ) or DMPP-stabilized ammonium. Microbial inoculants: Trichoderma harzianum T22 (Trianum P), Pseudomonas sp. DSMZ 13134 (Proradix), Trichoderma harzianum OMG16 + 5 Bacillus strains (Combifector-A); Bacillus amyloliquefaciens FZB42 (Rhizovital), Paenibacillus mucilaginosus , Penicillium sp. PK 112 (BFOD), Vitalin SP11 (VitSP11), or no inoculation (NoBE). Means of five replicates. One-way ANOVA, Tukey test. Different letters indicate significant differences ( p < 0.05); * indicates significant differences after pairwise comparison of PSM-inoculated variants versus the non-inoculated control with ammonium fertilization ( t -test, p < 0.05).

Article Snippet: Plants were inoculated with different PSMs of fungal and bacterial origin, comprising three single-strain inoculants ( Pseudomonas sp. DSMZ 13134 Proradix ® (Pro), Paenibacillus mucilaginosus (Paeni), Penicillium sp. PK 112, Biological Fertilizer OD (BFOD) and one consortium product, Vitalin SP11, with a combination of Bacillus subtilis , Pseudomonas sp., Streptomyces spp., humic acids, and extract of the seaweed, Ascophyllum nodosum .

Techniques: Comparison, Control

Journal: Microorganisms

Article Title: The Form of N Supply Determines Plant Growth Promotion by P-Solubilizing Microorganisms in Maize

doi: 10.3390/microorganisms7020038

Figure Lengend Snippet: Mineral nutritional status of maize (cv Colisee) grown on a clay-loam, organic farming soil (pH 7.0), supplied with and without (No P) P fertilization in the form of Rock-P or soluble triple superphosphate (TSP) and N supply in the form of Ca-nitrate (NO 3 ) or DMPP-stabilized ammonium (NH 4 ) as affected by different PSM inoculants ( Figure 3 ).

Article Snippet: Plants were inoculated with different PSMs of fungal and bacterial origin, comprising three single-strain inoculants ( Pseudomonas sp. DSMZ 13134 Proradix ® (Pro), Paenibacillus mucilaginosus (Paeni), Penicillium sp. PK 112, Biological Fertilizer OD (BFOD) and one consortium product, Vitalin SP11, with a combination of Bacillus subtilis , Pseudomonas sp., Streptomyces spp., humic acids, and extract of the seaweed, Ascophyllum nodosum .

Techniques: Concentration Assay